Visual demonstration of transmission electron microscopy for intracellular observation of a single bacterial cell
نویسندگان
چکیده
منابع مشابه
Transmission electron microscopy of the bacterial nucleoid.
Water-containing biological material cannot withstand the vacuum of the transmission electron microscope. The classical solution to this problem has been to dehydrate chemically fixed biological samples and then embed them in resin. During such treatment, the bacterial nucleoid is especially prone to aggregation, which affects its global shape and fine structure. Initial attempts to deal with a...
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Biological molecules are composed of light elements and can be considered as phase objects for the electron waves. Therefore, images of their unstained specimens show low contrast in transmission electron microscopy (TEM). Phase contrast TEM (P-TEM) is one of the powerful techniques to enhance image contrast of the phase objects [1]. In P-TEM, the phase plate (PP) is placed at a back focal plan...
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Noriyuki Ishii 1 Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central-6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan 2 Life Science Division, Lawrence Berkeley National Laboratory; Molecular and Cell Biology Department, University of California, Berkeley, California 94720, USA 3 Department of Biotechnology and Life Science, ...
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Bacteria can possess an outermost assembly of polysaccharide molecules, a capsule, which is attached to their cell wall. We have used two complementary, high-resolution microscopy techniques, atomic force microscopy (AFM) and transmission electron microscopy (TEM), to study bacterial capsules of four different gram-negative bacterial strains: Escherichia coli K30, Pseudomonas aeruginosa FRD1, S...
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ژورنال
عنوان ژورنال: Bangladesh Journal of Pharmacology
سال: 2017
ISSN: 1991-0088
DOI: 10.3329/bjp.v12i1.31390